Techno Press


Advances in Environmental Research   Volume 1, Number 2, June 2012, pages 97-108
DOI: http://dx.doi.org/10.12989/aer.2012.1.2.097
 
Effect of cyclodextrin glucanotransferase enzyme in biodegradation of diesel oil
Sivaraman C, Anasuya Ganguly and Srikanth Mutnuri

 
Abstract     [Full Text]
    Microbial degradation of hydrocarbons is found to be an attractive process for remediation of contaminated habitats. However the poor bioavailability of hydrocarbons results in low biodegradation rates. Cyclodextrins are known to increase the bioavailability of variety of hydrophobic compounds. In the present work we purified the Cyclodextrin Glucanotransferase (CGTase) enzyme which is responsible for converting starch into cyclodextrins and studied its role on biodegradation of diesel oil contaminated soil. Purification of CGTase from Enterobacter cloacae was done which resulted in 6 fold increase in enzyme activity. The enzyme showed maximum activity at pH 7, temperature 60
 
Key Words
    diesel oil; bioavailability; cyclodextrin; cylodextrin glucanotransferase
 
Address
Sivaraman C, Anasuya Ganguly and Srikanth Mutnuri: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS Pilani - K.K. Birla Goa Campus, Zuari Nagar, Goa 403726, India
 

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